An ultramicro fluorimetric determination of total ascorbic acid in human serum using 1,2-diamino-4,5-dimethoxybenzene.
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چکیده
منابع مشابه
Fluorimetric determination of total ascorbic acid by a stopped-flow mixing technique.
A simple, rapid and automatic fluorimetric method for the determination of total ascorbic acid is described. The method makes use of the stopped-flow mixing technique in order to achieve the rapid oxidation of ascorbic acid by dissolved oxygen to dehydroascorbic acid, which then reacts with o-phenylenediamine to form a fluorescent quinoxaline. The initial rate and fluorescence signal of this sy...
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A simple reproducible method for the ultramicro determination of total and direct serum bilirubin is presented. This method provides a greater sensitivity than that obtained with other colorimetric ultramicro procedures. Total bilirubin values agree with those obtained with the macro Malloy-Evelyn procedure, but direct-reading bilirubin values were significantly higher with the presented method.
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the total vitamin c content in human plasma is widely accepted as an indicator of the tissue status of vitamin c. a liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. a c18 reversed-phase column and cetrimide as an ion-pairing agent was employed. ascorbic acid (aa) was measured after reducing l-dehydroascorbic acid to l-asc...
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required for the prevention of deficiency diseases such as scurvy, it also might be useful for reducing the risk of developing chronic diseases such as cancer, cardiovascular disorders, and cataracts. The protective mechanism probably is due to the antioxidant effect of ascorbic acid (10). Therefore, applications of various detection systems and analytical procedures for the separation of ascor...
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A simple technique has been described by Wigglesworth (1937) for the determination of chloride in as little as 0-3 ,ul. of fluid. This is based on the Volhard titration and has been used in this Laboratory since 1947 for the examination of fish and crustacean blood. While the results obtained were satisfactory, the tedious back titration and the comparatively impermanent end point were found to...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1985
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.33.3499